FROM mmassaviol/mbb_workflows_base:latest COPY files /workflow COPY sagApp /sagApp RUN cd /opt/biotools \ && wget http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic/Trimmomatic-0.38.zip \ && unzip Trimmomatic-0.38.zip \ && echo -e '#!/bin/bash java -jar /opt/biotools/Trimmomatic-0.38/trimmomatic-0.38.jar' > bin/trimmomatic \ && chmod 777 bin/trimmomatic \ && rm Trimmomatic-0.38.zip RUN apt install -y fastqc=0.11.5+dfsg-6 RUN cd /opt/biotools \ && wget https://github.com/pachterlab/kallisto/releases/download/v0.45.0/kallisto_linux-v0.45.0.tar.gz \ && tar -zxvf kallisto_linux-v0.45.0.tar.gz \ && mv kallisto_linux-v0.45.0/kallisto bin \ && rm -r kallisto_linux-v0.45.0.tar.gz kallisto_linux-v0.45.0 RUN cd /opt/biotools \ && wget https://github.com/COMBINE-lab/salmon/releases/download/v1.0.0/salmon-1.0.0_linux_x86_64.tar.gz \ && tar -zxvf salmon-1.0.0_linux_x86_64.tar.gz \ && cd bin && ln -s ../salmon-latest_linux_x86_64/bin/salmon salmon \ && cd .. && rm -r salmon-1.0.0_linux_x86_64.tar.gz salmon-latest_linux_x86_64/sample_data.tgz RUN Rscript -e 'BiocManager::install("limma", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("edgeR", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("tximport", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("rhdf5", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("GenomicFeatures", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'install.packages("pheatmap",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("DESeq2", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("apeglm", version = "3.8",Ncpus=8, clean=TRUE)' RUN Rscript -e 'BiocManager::install("BiocParallel", version = "3.8",Ncpus=8, clean=TRUE)' #This part is necessary to run on ISEM cluster RUN mkdir -p /share/apps/lib \ && mkdir -p /share/apps/gridengine \ && mkdir -p /share/bio \ && mkdir -p /opt/gridengine \ && mkdir -p /export/scrach \ && mkdir -p /usr/lib64 \ && ln -s /bin/bash /bin/mbb_bash \ && ln -s /bin/bash /bin/isem_bash \ && /usr/sbin/groupadd --system --gid 400 sge \ && /usr/sbin/useradd --system --uid 400 --gid 400 -c GridEngine --shell /bin/true --home /opt/gridengine sge EXPOSE 3838 CMD ["Rscript", "-e", "setwd('/sagApp/'); shiny::runApp('/sagApp/app.R',port=3838 , host='0.0.0.0')"]