diff --git a/03_dereplication/test/test_derep_tally.sh b/03_dereplication/test/test_derep_tally.sh
index 91487114c13f9972be6140004288e6ddf6956c6c..49f2e7317370c2baee12f5bfce112355136a8363 100644
--- a/03_dereplication/test/test_derep_tally.sh
+++ b/03_dereplication/test/test_derep_tally.sh
@@ -3,4 +3,7 @@ tally=${SINGULARITY_EXEC_CMD}" "${EDNATOOLS_SIMG}" tally"
 ALL_SAMPLE_SEQ_UNIQ=$(pwd)/03_dereplication/test/01_vsearch/grinder_teleo1_all_sample_clean.uniq.fasta
 
 
-$tally --fasta-in --fasta-out -i ${ALL_SAMPLE_SEQ_UNIQ} -o test
\ No newline at end of file
+$tally --fasta-in --fasta-out -i ${ALL_SAMPLE_SEQ_UNIQ} -o test
+
+$obigrep -s '^[ACGT]+$' -l 20 test > test20
+