diff --git a/04_filtering/filter_prinseq.sh b/04_filtering/filter_prinseq.sh index 3dfc7756b296894b89a12bcfd0bb5bb9ec64d130..5798298f473d83a4b0ed06660b23d866cfdc416e 100644 --- a/04_filtering/filter_prinseq.sh +++ b/04_filtering/filter_prinseq.sh @@ -32,7 +32,7 @@ ecotag=${SINGULARITY_EXEC_CMD}" "${OBITOOLS_SIMG}" ecotag" obisort=${SINGULARITY_EXEC_CMD}" "${OBITOOLS_SIMG}" obisort" obitab=${SINGULARITY_EXEC_CMD}" "${OBITOOLS_SIMG}" obitab" ## EDNAtools -prinseq=${SINGULARITY_EXEC_CMD}" "${EDNATOOLS_SIMG}" prinseq" +prinseq=${SINGULARITY_EXEC_CMD}" "${EDNATOOLS_SIMG}" perl prinseq-lite-0.20.4/prinseq-lite.pl" container_python2=${SINGULARITY_EXEC_CMD}" "${EDNATOOLS_SIMG}" python2" @@ -81,11 +81,12 @@ do dereplicated_sample="${sample/.fasta/.uniq.fasta}" echo "$obiuniq -m sample "$sample" > "$dereplicated_sample > $sample_sh; # Keep only sequences longer than 20pb with no N bases - good_sequence_sample="${dereplicated_sample/.fasta/.l20.fasta}" + ### be careful! prinseq will add .fasta as suffix output + good_sequence_sample="${dereplicated_sample/.fasta/.l20}" echo "/usr/bin/time $prinseq -fasta "$dereplicated_sample" -min_len 20 -ns_max_n 0 -noniupac --out_format 1 -out_good "$good_sequence_sample >> $sample_sh # Removal of PCR and sequencing errors (variants) - clean_sequence_sample="${good_sequence_sample/.fasta./.r005.clean.fasta}" - echo "$obiclean -r 0.05 -H "$good_sequence_sample" > "$clean_sequence_sample >> $sample_sh + clean_sequence_sample="${good_sequence_sample}".r005.clean.fasta + echo "$obiclean -r 0.05 -H "$good_sequence_sample".fasta > "$clean_sequence_sample >> $sample_sh done parallel < $all_samples_parallel_cmd_sh # Concatenate all files into one main file @@ -113,5 +114,3 @@ all_sample_sequences_sort="${all_sample_sequences_ann/.fasta/.sort.fasta}" $obisort -k count -r $all_sample_sequences_ann > $all_sample_sequences_sort # Create the final table $obitab -o $all_sample_sequences_sort > $fin_dir/"$step".csv - -gzip $main_dir/* \ No newline at end of file diff --git a/04_filtering/test/04_prinseq/grinder_teleo1_sample_S15-11.uniq.l20.fasta b/04_filtering/test/04_prinseq/grinder_teleo1_sample_S15-11.uniq.l20.fasta new file mode 100644 index 0000000000000000000000000000000000000000..a79939264dba6bcb85bdbf54de34d12b75001d14 --- /dev/null +++ b/04_filtering/test/04_prinseq/grinder_teleo1_sample_S15-11.uniq.l20.fasta @@ -0,0 +1,1252 @@ +>11_1/1_CONS_SUB_SUB merged_sample={'S15-11': 12382}; status=full; direction=forward; count=12382; seq_length=62; experiment=S15-11; mode=alignment; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_2/1_CONS_SUB_SUB reverse_score=74.0; merged_sample={'S15-11': 2340}; direction=forward; reverse_match=cttccggtacacttaccgtg; seq_length_ori=117; count=2340; seq_length=64; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_4/1_CONS_SUB_SUB merged_sample={'S15-11': 8867}; status=full; direction=forward; forward_tag=attcgcag; count=8867; seq_length=60; experiment=S15-11; seq_a_insertion=0; mode=alignment; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_6/1_CONS_SUB_SUB reverse_score=80.0; ali_length=99; direction=forward; seq_a_insertion=0; experiment=S15-11; seq_b_deletion=0; forward_score=68.0; reverse_match=cttccggtacacttaccatg; seq_length_ori=115; count=89; seq_length=62; reverse_tag=attcgcag; merged_sample={'S15-11': 89}; status=full; mode=alignment; forward_tag=attcgcag; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +ccccaagttcttatacattaataactaatacctcaccagaacaaaggggaggcaagtcgt +aa +>11_11/1_CONS_SUB_SUB merged_sample={'S15-11': 1655}; status=full; direction=forward; count=1655; seq_length=62; experiment=S15-11; seq_a_insertion=0; mode=alignment; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +ccccaataaacgaataaaaataactaataatataacactaattaaggggagataagtcgt +aa +>11_12/1_CONS_SUB_SUB reverse_score=80.0; merged_sample={'S15-11': 6849}; direction=forward; reverse_match=cttccggtacacttaccatg; seq_length_ori=124; count=6849; seq_length=71; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_14/1_CONS_SUB_SUB merged_sample={'S15-11': 4698}; status=full; direction=forward; forward_tag=attcgcag; count=4698; seq_length=61; experiment=S15-11; mode=alignment; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_19/1_CONS_SUB_SUB merged_sample={'S15-11': 1127}; status=full; direction=forward; forward_tag=attcgcag; count=1127; seq_length=64; experiment=S15-11; seq_a_insertion=0; mode=alignment; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +cccctgtcacacagcaacaaatgtccataacacccaagcactgacaaggggaggcaagtc +gtaa +>11_28/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_56/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgctttcaggaacaaaggggaggcaagtcgta +a +>11_62/1_CONS_SUB_SUB reverse_score=80.0; ali_length=105; direction=forward; seq_b_insertion=0; seq_a_insertion=0; experiment=S15-11; forward_score=68.0; reverse_match=cttccggtacacttaccatg; seq_length_ori=121; count=97; seq_length=68; reverse_tag=attcgcag; merged_sample={'S15-11': 97}; status=full; mode=alignment; forward_tag=attcgcag; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +ccccccaccccaccttaaacctaacattcctaataacacaaacctaaattcggggaggca +agtcgtaa +>11_83/1_CONS_SUB_SUB merged_sample={'S15-11': 861}; status=full; direction=forward; forward_tag=attcgcag; count=861; seq_length=63; experiment=S15-11; seq_a_insertion=0; mode=alignment; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +ccccgacatagcggataaattttaaataaacaataatccaggataaggggaggaaagtcg +taa +>11_84/1_CONS_SUB_SUB reverse_score=74.0; merged_sample={'S15-11': 237}; direction=forward; seq_a_insertion=0; reverse_match=cttccggtacgcttaccatg; seq_length_ori=119; count=237; seq_length=66; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_b_single=8; +ccccaagcaactggacctaaaattttctttaaaccctaacaaccgcgaaggagaggaaag +tcgtaa +>11_87/1_CONS_SUB_SUB reverse_score=74.0; merged_sample={'S15-11': 623}; direction=forward; seq_a_insertion=0; reverse_match=cttccggtacgcttaccatg; seq_length_ori=119; count=623; seq_length=66; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +caccaagcatctgaacaactctatcttaagccccgttcaatacgcacaaggggaggaaag +tcgtaa +>11_91/1_CONS_SUB_SUB merged_sample={'S15-11': 3296}; status=full; direction=forward; forward_tag=attcgcag; count=3296; seq_length=63; experiment=S15-11; mode=alignment; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_98/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagttttcaggaacaaaggggaggcaagtcg +taa +>11_101/1_CONS_SUB_SUB ali_length=98; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 136}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=136; seq_length=61; status=full; mode=alignment; seq_b_single=8; +ctccaagtttaacacccttttaaataaaaaattaacagaacaaaggagaggcaagtcgta +a +>11_108/1_CONS_SUB_SUB reverse_score=80.0; ali_length=99; direction=forward; seq_a_insertion=0; experiment=S15-11; forward_score=62.0; reverse_match=cttccggtacacttaccatg; seq_length_ori=115; count=426; seq_length=62; reverse_tag=attcgcag; merged_sample={'S15-11': 426}; status=full; mode=alignment; forward_tag=attcgcag; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +cctcgactcgataaaaatatattcttaaacaaagatagaactaaagaggaggcaagtcgt +aa +>11_143/1_CONS_SUB_SUB ali_length=97; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 13}; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=13; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_152/1_CONS_SUB_SUB reverse_score=74.0; ali_length=101; direction=forward; seq_a_insertion=0; experiment=S15-11; forward_score=68.0; reverse_match=cttccggtacacttaccgtg; seq_length_ori=117; count=310; seq_length=64; reverse_tag=attcgcag; merged_sample={'S15-11': 310}; status=full; mode=alignment; forward_tag=attcgcag; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +cccctgtcaaaacgcataagaaatacataaccaaccagcactgacaaggggaggcaagtc +gtaa +>11_199/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.6538461538; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctgtcacacagcaacaaatgtccataacacccaagcactgacaaggggaggcgggag +gcaagtcgtaa +>11_236/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 4}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=4; seq_length=61; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +g +>11_251/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattccttcgatggcaaaggagagggaagtcgt +aa +>11_288/1_CONS_SUB_SUB ali_length=108; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=423.575366834; mid_quality=71.5961538462; avg_quality=67.2741935484; seq_a_single=8; score_norm=3.92199413735; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacgttcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_297/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaaaaaggggaggcaagtcgta +a +>11_342/1_CONS_SUB_SUB reverse_score=74.0; merged_sample={'S15-11': 17}; direction=forward; seq_b_insertion=0; seq_a_insertion=0; reverse_match=cttccggtacacttaccgtg; seq_length_ori=116; seq_a_deletion=0; count=17; seq_length=63; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; +ccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_389/1_CONS_SUB_SUB ali_length=101; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 48}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=48; seq_length=64; status=full; mode=alignment; seq_b_single=8; +cccctgtcaacacgcaataaagattcataacaataaagcactgacaaggggaggcaagtc +gtaa +>11_391/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=371.624245684; mid_quality=70.8631578947; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttaagagggaagtcgt +aa +>11_478/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgggcaaaggagagggaagtcgt +aa +>11_490/1_CONS_SUB_SUB ali_length=101; tail_quality=44.8; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=2; seq_length=64; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaagaggggaggcaagtc +gtaa +>11_546/1_CONS_SUB_SUB ali_length=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=3; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacaatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_636/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ccccgactccgaacttaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_639/1_CONS_SUB_SUB ali_length=101; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 15}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=15; seq_length=64; status=full; mode=alignment; seq_b_single=8; +ccccgagcttacaaacacgaatatacataaaatgcttaaaccgctaaggggagacaagtc +gtaa +>11_677/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; seq_b_single=8; +ctccgaattcagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_705/1_CONS_SUB_SUB ali_length=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 6}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=6; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcaacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_736/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gc +>11_878/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=1; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.601501083; mid_quality=71.6288659794; avg_quality=67.0427350427; seq_a_single=8; score_norm=3.83763862458; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaacaaggggaggcaagtc +gtaa +>11_885/1_CONS_SUB_SUB reverse_score=80.0; merged_sample={'S15-11': 55}; direction=forward; seq_a_insertion=0; reverse_match=cttccggtacacttaccatg; seq_length_ori=123; seq_a_deletion=0; count=55; seq_length=70; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; +ccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_945/1_CONS_SUB_SUB ali_length=98; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_960/1_CONS_SUB_SUB ali_length=101; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 60}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=60; seq_length=64; status=full; mode=alignment; seq_b_single=8; +cccctgtcgtaatgcaatgaagttacctaacaccaaagctctgacaaggggaggcaagtc +gtaa +>11_980/1_CONS_SUB_SUB ali_length=99; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=64.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=3; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggggagataagtcgtaa +>11_1001/1_CONS_SUB_SUB ali_length=99; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=395.59021283; mid_quality=71.8736842105; avg_quality=67.1652173913; seq_a_single=8; score_norm=3.99586073566; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagttgtaa +>11_1263/1_CONS_SUB_SUB reverse_score=80.0; ali_length=98; direction=forward; seq_b_insertion=0; seq_a_insertion=0; experiment=S15-11; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; count=49; seq_length=61; merged_sample={'S15-11': 49}; status=full; mode=alignment; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_b_single=8; +tccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_1353/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggta +a +>11_1475/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagcacggggaggc +aagtcgtaa +>11_1576/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaaccttcttaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_1625/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattccttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_1679/1_CONS_SUB_SUB ali_length=108; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=399.595434532; mid_quality=70.6153846154; avg_quality=66.4516129032; seq_a_single=8; score_norm=3.69995772715; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtcattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_1697/1_CONS_SUB_SUB reverse_score=74.0; ali_length=98; direction=forward; seq_a_insertion=0; experiment=S15-11; seq_b_deletion=1; reverse_match=cttccggtacgcttaccatg; seq_length_ori=114; seq_a_deletion=0; count=24; seq_length=59; head_quality=44.8; merged_sample={'S15-11': 24}; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +caagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_1958/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaaccaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_2007/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=407.568755633; mid_quality=71.1923076923; avg_quality=66.935483871; seq_a_single=8; score_norm=3.77378477438; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaacctcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_2205/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.605519428; mid_quality=71.625; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgcgtaatgcaatgaagttacctaacaccaaagctctgacaaggggaggcaagtcg +taa +>11_2244/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttactacg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +catggtaag +>11_2280/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcgactcgataaaaatatattcttaaacaaacaaccgaacaaaggggaggcgcaagtc +gtaa +>11_2478/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; score=375.613556119; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagctagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_2568/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggagagggaagtcgt +aa +>11_2575/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.6538461538; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +catggtaaa +>11_2620/1_CONS_SUB_SUB ali_length=103; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=387.608172303; mid_quality=71.2727272727; avg_quality=66.5210084034; seq_a_single=8; score_norm=3.76318613886; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=119; reverse_tag=attcgcag; count=1; seq_length=66; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccgagcatctgaacaactctatcttaagccccgttcaatacgcacaaggggaggaaag +tcgtaa +>11_2720/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgagcttagtactaaacctaactaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_2777/1_CONS_SUB_SUB ali_length=98; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 12}; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=12; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccaataaacgaataaaaataactaataatataacactaattaaggggagataagtcgta +a +>11_2827/1_CONS_SUB_SUB ali_length=99; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 19}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=19; seq_length=62; status=full; mode=alignment; seq_b_single=8; +ccccaagccaacatcatcctataaataatacattttaccggtaaaggggaggcaagtcgt +aa +>11_2850/1_CONS_SUB_SUB ali_length=98; seq_ab_match=95; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=359.628264029; mid_quality=71.2340425532; avg_quality=66.2807017544; seq_a_single=8; score_norm=3.66967616356; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_2871/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttgtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_2889/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacatttatcagcacggggag +gcaagtcgtaa +>11_2900/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=371.624245684; mid_quality=70.8631578947; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaagcaactggacctaaaattttctttaaattcgatggcaaaggagagggaagtcgt +aa +>11_2956/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_3047/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaaggaggcaagtcgta +a +>11_3050/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccaataactaataatataacataattaaggggagataagtcgta +a +>11_3062/1_CONS_SUB_SUB reverse_score=80.0; ali_length=99; direction=forward; seq_a_insertion=0; experiment=S15-11; seq_b_deletion=1; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_length_ori=115; seq_a_deletion=0; count=19; seq_length=62; merged_sample={'S15-11': 19}; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +cccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcgt +aa +>11_3212/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcgt +aa +>11_3213/1_CONS_SUB_SUB ali_length=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; mid_quality=71.64; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=120; reverse_tag=attcgcag; count=2; seq_length=67; status=full; mode=alignment; seq_b_single=8; +ccccccacccaccttaaacctaacattcctaataacacaaacctaaattcggggaggcaa +gtcgtaa +>11_3271/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttctaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_3274/1_CONS_SUB_SUB ali_length=107; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=419.570037056; mid_quality=72.0; avg_quality=67.5772357724; seq_a_single=8; score_norm=3.92121529959; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattctaattaaacatttatcagcacggggagg +caagtcgtaa +>11_3496/1_CONS_SUB_SUB merged_sample={'S15-11': 5}; forward_score=64.0; direction=forward; seq_b_insertion=0; seq_a_insertion=0; tail_quality=44.8; forward_tag=attcgcag; seq_a_deletion=0; count=5; seq_length=58; head_quality=44.8; experiment=S15-11; status=full; mode=alignment; seq_b_deletion=1; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +caaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_3545/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgg +gcaagtcgtaa +>11_3724/1_CONS_SUB_SUB ali_length=99; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=387.602183818; mid_quality=72.0; avg_quality=67.2695652174; seq_a_single=8; score_norm=3.91517357392; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatgcggataaattttaaataaacaataatccaggataaggggaggaaagtcgt +aa +>11_3802/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaacaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_3853/1_CONS_SUB_SUB reverse_score=74.0; merged_sample={'S15-11': 10}; direction=forward; seq_b_insertion=0; seq_b_deletion=0; forward_score=56.0; reverse_match=cttccggtacgcttaccatg; seq_length_ori=117; seq_a_deletion=0; count=10; seq_length=64; reverse_tag=attcgcag; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_b_single=8; +caccaagcccccagacagccgtaattaacaagcctgacatgtgcgaaggggaggaaagtc +gtaa +>11_3869/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.605519428; mid_quality=72.0; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccctgtcgtaatgcaatgaagttacctaacaccaaagctctgacaaggggaggcaagtcg +taa +>11_3927/1_CONS_SUB_SUB ali_length=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_3993/1_CONS_SUB_SUB status=full; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=3; seq_length=70; mode=alignment; seq_b_single=8; +cccctcacacatcaaactcaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_4053/1_CONS_SUB_SUB ali_length=97; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=371.617574464; mid_quality=71.6129032258; avg_quality=66.8672566372; seq_a_single=8; score_norm=3.83110901509; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=2; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_4058/1_CONS_SUB_SUB ali_length=107; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=403.584744967; mid_quality=71.3009708738; avg_quality=66.9918699187; seq_a_single=8; score_norm=3.77182004642; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaataaacatttatcagcacggggagg +caagtcgtaa +>11_4069/1_CONS_SUB_SUB ali_length=101; seq_ab_match=97; tail_quality=44.8; reverse_match=cttcaggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=371.623562949; mid_quality=70.5154639175; avg_quality=66.1196581197; seq_a_single=8; score_norm=3.67944121731; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcctaacacccaaaaaacatttatctaaaacctacttgaccgtagaggagaggcaagtc +gtaa +>11_4108/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.611531941; mid_quality=71.5578947368; avg_quality=66.9043478261; seq_a_single=8; score_norm=3.91526799941; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataagcgaataaaaataactaataatataacactaattaaggggagataagtcgt +aa +>11_4144/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcag +gc +>11_4162/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +gcaagtcgtaa +>11_4178/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctatatctaatataatagcactgacaaggggaggcaagtcgt +aa +>11_4292/1_CONS_SUB_SUB ali_length=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 8}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=8; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_4302/1_CONS_SUB_SUB ali_length=97; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=371.617574464; mid_quality=71.6129032258; avg_quality=66.8672566372; seq_a_single=8; score_norm=3.83110901509; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_4379/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=395.601501083; mid_quality=71.6288659794; avg_quality=67.0427350427; seq_a_single=8; score_norm=3.91684654538; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaaggggaggcaagtc +gtaa +>11_4443/1_CONS_SUB_SUB ali_length=99; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=395.59021283; mid_quality=71.8736842105; avg_quality=67.1652173913; seq_a_single=8; score_norm=3.99586073566; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttctcaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_4521/1_CONS_SUB_SUB reverse_score=80.0; merged_sample={'S15-11': 14}; direction=forward; seq_b_insertion=0; seq_a_insertion=0; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; count=14; seq_length=60; head_quality=44.8; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; reverse_tag=attcgcag; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +ccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_4584/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaacgcataagaaatacataaccaacaccgaacaaaggggaggcaagtcgt +aa +>11_4698/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=391.607513596; mid_quality=71.5625; avg_quality=66.9482758621; seq_a_single=8; score_norm=3.91607513596; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggagacaagtcg +taa +>11_4777/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=3; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_4782/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaggataaggggaggaaagtcg +taa +>11_4785/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=3; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggagcacggggag +gcaagtcgtaa +>11_4924/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; score=371.624245684; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; experiment=S15-11; mid_quality=70.8631578947; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +ta +>11_4991/1_CONS_SUB_SUB ali_length=99; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_5026/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtaaactcaaaccaaacattcttaattaaacatttatcagcacggggaggc +aagtcgtaa +>11_5050/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaaatcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_5253/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=41.2; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=367.620910074; mid_quality=71.6170212766; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.75123377627; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +tccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgta +a +>11_5317/1_CONS_SUB_SUB ali_length=101; seq_ab_match=98; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616208993; mid_quality=71.2577319588; avg_quality=66.4273504274; seq_a_single=8; score_norm=3.75857632667; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgacactgacaaggggaggcaagtcgt +aa +>11_5368/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=395.603495251; mid_quality=71.5670103093; avg_quality=66.9914529915; seq_a_single=8; score_norm=3.91686628961; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatctaatagcactgacaaggggaggcaagtc +gtaa +>11_5383/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_5397/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtctc +gtaa +>11_5425/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 4}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=4; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccccagccaacaacatcctataaataatacattctaccggtaaaggggaggcaagtcgt +aa +>11_5433/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.6170212766; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacatcgcttcgatggcaaagggaggcaagtcgta +a +>11_5479/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaatttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_5555/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 4}; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=4; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_5565/1_CONS_SUB_SUB ali_length=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; mid_quality=71.3009708738; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; seq_b_single=8; +cccctcaccatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_5567/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=2; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aaaa +>11_5676/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgagcaaaggagagggaagtcgt +aa +>11_5823/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.6288659794; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccgaattcacttaatctggtttatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_5839/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_5875/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=375.622221507; mid_quality=70.8125; avg_quality=66.3275862069; seq_a_single=8; score_norm=3.75622221507; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagtataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_5939/1_CONS_SUB_SUB ali_length=100; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 6}; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=6; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccctgtcacacagcaacaaatgtccataacacccaagcactgacaaggggaggcaagtcg +taa +>11_5998/1_CONS_SUB_SUB ali_length=98; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 7}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=7; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_6091/1_CONS_SUB_SUB ali_length=107; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=411.577391011; mid_quality=71.6504854369; avg_quality=67.2845528455; seq_a_single=8; score_norm=3.846517673; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacattatcagcacggggagg +caagtcgtaa +>11_6113/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagcttcgatggcaaaggagagggaagtcgt +aa +>11_6122/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.582720789; mid_quality=71.25; avg_quality=66.9838709677; seq_a_single=8; score_norm=3.84798815546; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaccattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_6129/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacaaccaaacattcttaattaaacatttatcagcacggggaggc +aagtcgtaa +>11_6251/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.6538461538; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagataattaaacatttatcagcacggggag +gcaagtcgtaa +>11_6577/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 5}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=5; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaatcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_6601/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=71.3076923077; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +catgcgtaa +>11_6615/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacattggggaggcaagtcgt +aa +>11_6627/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; seq_b_single=8; +ctccgaattcacttaatctggttcttaacacgcttcgatggcaaaggagagggaagtcgt +aa +>11_6683/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgataacaaaggggaggcaagtcgtaa +>11_6756/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgacaaccgaacaaaggggaggcaagtcgtaa +>11_6758/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggcacggggag +gcaagtcgtaa +>11_6821/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaacatttatcagcacggggag +gcaagtcgtaa +>11_7118/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggggaggcaagtcgtaa +>11_7250/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=41.2; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaactcgatggcaaaggagagggaagtcgt +aa +>11_7406/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcactaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_7425/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_7532/1_CONS_SUB_SUB ali_length=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 10}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=10; seq_length=63; status=full; mode=alignment; seq_b_single=8; +ctccgaaccaacaactttcccaaatttataatacacttttccggtaaagggagcaagtcg +taa +>11_7688/1_CONS_SUB_SUB ali_length=99; seq_ab_match=95; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=363.631599639; mid_quality=70.4842105263; avg_quality=66.0173913043; seq_a_single=8; score_norm=3.673046461; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacgttcttaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_7700/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaagatcagcaagtcgtaa +>11_7781/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactattaaggggagataagtcgta +a +>11_8019/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=371.626239852; mid_quality=71.1789473684; avg_quality=66.2782608696; seq_a_single=8; score_norm=3.75380040254; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggctagtcgt +aa +>11_8083/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.6538461538; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttaaataaacaataatccaggataaggcagcacggggag +gcaagtcgtaa +>11_8097/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620227339; mid_quality=71.625; avg_quality=66.3793103448; seq_a_single=8; score_norm=3.75620227339; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctcatccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_8146/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaaaaacatttatcagcacggggag +gcaagtcgtaa +>11_8288/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacagtcgt +aa +>11_8298/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgta +a +>11_8360/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaacacggta +>11_8444/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_8474/1_CONS_SUB_SUB merged_sample={'S15-11': 15}; forward_score=68.0; direction=forward; seq_b_insertion=0; seq_a_insertion=0; forward_tag=attcgcag; seq_a_deletion=0; count=15; seq_length=61; reverse_tag=attcgcag; experiment=S15-11; status=full; mode=alignment; seq_b_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; seq_b_single=8; +cctcaacataaaattcccaatatataaataaactatttccaaaagaggagacaagtcgta +a +>11_8613/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_8651/1_CONS_SUB_SUB ali_length=107; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=2; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaacatttatcagcacggggagg +caagtcgtaa +>11_8678/1_CONS_SUB_SUB status=full; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=3; seq_length=63; mode=alignment; head_quality=44.8; +cccctgtcaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_8899/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 6}; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=6; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccgacatagcggataaattttaaataaacaataatccaggataaggggaggaaagtcgt +aa +>11_8941/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; seq_b_mismatch=2; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +catgggtaa +>11_8944/1_CONS_SUB_SUB ali_length=102; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=391.604836693; mid_quality=71.2653061224; avg_quality=66.7796610169; seq_a_single=8; score_norm=3.83926310483; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=118; reverse_tag=attcgcag; count=2; seq_length=65; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaacaaccgcctacaccaaggtaaataacacaacatccgtcacaaggggaggcaagt +cgtaa +>11_8993/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_9059/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.605519428; mid_quality=71.625; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctatataatagcactgacaaggggaggcaagtcg +taa +>11_9097/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_9268/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaaccttcttaacatcttaattaaacatttatcagcacggggaggc +aagtcgtaa +>11_9400/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_9511/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaacaaaggggaggcaagtcgt +aa +>11_9533/1_CONS_SUB_SUB ali_length=107; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=411.577391011; mid_quality=71.6504854369; avg_quality=67.2845528455; seq_a_single=8; score_norm=3.846517673; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_9567/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattgcttcgatggcaaaggagagggaagtcgt +aa +>11_9618/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgccttaacaccaagaggaggcaagtcgtaa +>11_9660/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaattaaggggagataagtcgt +gcaagtcgtaa +>11_9699/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_9802/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_9834/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_9840/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=371.624245684; mid_quality=71.2421052632; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +caccaagcatctgaacaactctatcttaagccccgttcaatacgcacaagggcaagtcgt +aa +>11_9949/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=41.2; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=375.612873383; mid_quality=71.625; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.75612873383; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatcaccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_9958/1_CONS_SUB_SUB ali_length=99; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=395.59021283; mid_quality=71.8736842105; avg_quality=67.1652173913; seq_a_single=8; score_norm=3.99586073566; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagctgagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_9966/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttaaataaacaataatccaggaaaggggaggcaagtcgt +aa +>11_10087/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaaagacaaccgaacaaaggggaggcaagtcgtaa +>11_10139/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; score=379.616891729; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacgggcgt +aa +>11_10231/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=395.601501083; mid_quality=71.6288659794; avg_quality=67.0427350427; seq_a_single=8; score_norm=3.91684654538; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_10456/1_CONS_SUB_SUB ali_length=97; seq_ab_match=95; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=363.624928419; mid_quality=71.2258064516; avg_quality=66.5486725664; seq_a_single=8; score_norm=3.74871060226; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggagcaagtcgtaa +>11_10566/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=71.3076923077; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacacagcaacaaatgtccataacacccattaaacatttatcagcacggggag +gcaagtcgtaa +>11_10581/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaagggaggcaagtcgta +a +>11_10659/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaatggcaaaggagagggaagtcgt +aa +>11_10684/1_CONS_SUB_SUB ali_length=98; seq_ab_match=98; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=391.606202164; mid_quality=72.0; avg_quality=67.2280701754; seq_a_single=8; score_norm=3.99598165473; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaatcaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_10696/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.590074744; mid_quality=70.9038461538; avg_quality=66.6935483871; seq_a_single=8; score_norm=3.77398217356; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacagcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_10711/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaattggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_10772/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; seq_b_single=8; +cccctcattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_10827/1_CONS_SUB_SUB ali_length=99; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggagcaagtcgt +aa +>11_10840/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcctggacctaaaattttctttaaaccctaacaaccgcgaaggcaagtcgta +a +>11_10973/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=1; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.601501083; mid_quality=71.6288659794; avg_quality=67.0427350427; seq_a_single=8; score_norm=3.83763862458; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaacaaggggaggcaagtc +gtaa +>11_11589/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctacttaaacaaagatagaactaaagaggaggcaagtcgtaa +>11_11802/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacgggtcg +taacatggtaa +>11_11888/1_CONS_SUB_SUB ali_length=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 4}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=4; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaaaaaatatcaccctacttatataaaaccttagaacaataaaggggaggcaagtcg +taa +>11_11913/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; score=387.608855038; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; status=full; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=2; seq_length=64; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgactgacaaggggaggcaagtc +gtaa +>11_12182/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=0; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacaaccgaacaaaggggaggcaagtcgt +aa +>11_12333/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=41.2; reverse_match=cttccggtacaacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=371.624245684; mid_quality=71.2421052632; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=78.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacagcaacaaatgtccataacaccttaacaccaagaggaggcaagtcgta +a +>11_12449/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcgactcgaaaaaatatattcttaaacaaagatagaactaaagaggaggcaagtcgta +a +>11_12521/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggaaaggagagggaagtcgtaa +>11_12587/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttagggaggcaagtcg +taa +>11_12628/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aaa +>11_12659/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=1; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.75850351523; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgatcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_12736/1_CONS_SUB_SUB ali_length=107; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=403.584744967; mid_quality=71.3009708738; avg_quality=66.9918699187; seq_a_single=8; score_norm=3.77182004642; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaactttatcagcacggggagg +caagtcgtaa +>11_12875/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaactggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_13117/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=415.582720789; mid_quality=71.25; avg_quality=66.9838709677; seq_a_single=8; score_norm=3.84798815546; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaaccttcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_13172/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaaaggggaggcaagtcgt +aa +>11_13203/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aacatggtaaa +>11_13377/1_CONS_SUB_SUB ali_length=108; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttacacactt; seq_a_deletion=0; forward_match=ataccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=58.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=3; score=399.595434532; mid_quality=70.6153846154; avg_quality=66.4516129032; seq_a_single=8; score_norm=3.69995772715; reverse_score=70.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=67; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +acatggt +>11_13624/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaattaaggggaggcaagtcgt +aa +>11_13645/1_CONS_SUB_SUB ali_length=97; seq_ab_match=95; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=363.624928419; mid_quality=71.2258064516; avg_quality=66.5486725664; seq_a_single=8; score_norm=3.74871060226; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaatagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_13886/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaacaaatgtccataacacccaagcactgacaaggggaggcaagtc +gtaa +>11_13914/1_CONS_SUB_SUB ali_length=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctccacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_14067/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacagacaaccgaacaaaggggaggcaagtcgta +a +>11_14297/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaattaagggcagcacggggag +gcaagtcgtaa +>11_14339/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acccgcccgtcactctc; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=367.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.75123377627; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccaagttcttatacattaataactaatacctcaccagaacaaaggggaggcaagtcgta +a +>11_14615/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaacaagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_14647/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatctttcgatggcaaaggagagggaagtcgt +aa +>11_14677/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_14791/1_CONS_SUB_SUB ali_length=99; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 4}; forward_score=64.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=4; seq_length=60; status=full; mode=alignment; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagaggcaagtcgtaa +>11_14961/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaagatggcaaaggagagggaagtcgt +aa +>11_15000/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaaaaacgaataaaaataactaataatataacactaattaaggggagataagtcgta +a +>11_15208/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_15251/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagaaaccgaacaaaggggaggcaagtcgta +a +>11_15327/1_CONS_SUB_SUB ali_length=108; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=2; experiment=S15-11; mid_quality=70.9615384615; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaagggcagcacggggag +gcaagtcgtaa +>11_15423/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggagggcaagtcgt +aa +>11_15591/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatgacaaccgaacaaaggggaggcaagtcgt +aa +>11_15843/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.605519428; mid_quality=71.625; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacagcaacaaatgtccataacacccagcactgacaaggggaggcaagtcg +taa +>11_15949/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccaagtttaacacccttttaaataaaaaattaaaccgaacaaaggggaggcaagtcgt +aa +>11_15953/1_CONS_SUB_SUB ali_length=102; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.604836693; mid_quality=71.2653061224; avg_quality=66.7796610169; seq_a_single=8; score_norm=3.76083173228; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=118; reverse_tag=attcgcag; count=1; seq_length=65; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacaactctatcttaagccccgttcatacgcacaaggggaggaaagt +cgtaa +>11_16257/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=1; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=383.605519428; mid_quality=71.625; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacacaataatccaggataaggggaggaaagtcg +taa +>11_16300/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcgt +aa +>11_16369/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagagggaggcaagtcgt +aa +>11_16407/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcatctcct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=58.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=367.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.75123377627; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cgactcgataaaaatatattcttaaacaaagatagaactaaagaggaggcaagtcgtaa +>11_16533/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaatatcagcacggggaggc +aagtcgtaa +>11_16871/1_CONS_SUB_SUB ali_length=98; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=64.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaataaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_17244/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaaacaaccgaacaaaggggaggcaagtcgta +a +>11_17361/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_17394/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.576109588; mid_quality=71.1923076923; avg_quality=66.6451612903; seq_a_single=8; score_norm=3.77385286656; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaatcatttatcagcacggggag +gcaagtcgtaa +>11_17404/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggagggaagtcgt +aa +>11_17406/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaacttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_17443/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctgttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_17542/1_CONS_SUB_SUB ali_length=97; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=379.610220509; mid_quality=72.0; avg_quality=67.185840708; seq_a_single=8; score_norm=3.91350742793; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_17556/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggtcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_17726/1_CONS_SUB_SUB ali_length=107; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; score=403.584744967; mid_quality=71.3009708738; avg_quality=66.9918699187; seq_a_single=8; score_norm=3.77182004642; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacactcaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_17893/1_CONS_SUB_SUB ali_length=99; seq_ab_match=95; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=363.631599639; mid_quality=70.4842105263; avg_quality=66.0173913043; seq_a_single=8; score_norm=3.673046461; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttgggaggcaagtcgt +aa +>11_17912/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccgaattcacttaatctggttcctaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_18286/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcgcactgacaaggggaggcaagtc +gtaa +>11_18350/1_CONS_SUB_SUB ali_length=107; seq_ab_match=105; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=403.584744967; mid_quality=71.6504854369; avg_quality=66.9918699187; seq_a_single=8; score_norm=3.77182004642; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacataaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_18525/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=375.620227339; mid_quality=70.875; avg_quality=66.3793103448; seq_a_single=8; score_norm=3.75620227339; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaatttcaggaacaaaggggaggcaagtcg +taa +>11_18563/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_18644/1_CONS_SUB_SUB ali_length=107; seq_ab_match=106; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=411.577391011; mid_quality=72.0; avg_quality=67.2845528455; seq_a_single=8; score_norm=3.846517673; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_18647/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.568755633; mid_quality=71.1923076923; avg_quality=66.935483871; seq_a_single=8; score_norm=3.84785884846; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattattaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_19005/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctgctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_19072/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.75612873383; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaagcaccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_19348/1_CONS_SUB_SUB ali_length=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 6}; forward_score=62.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=6; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgtcaaaatacaccaaaaatacttaatacaataacactaacaaggggaggcaagtcg +taa +>11_19366/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=367.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.75123377627; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcagtcgta +a +>11_19498/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaagagagggaagtcgtaa +>11_19613/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=395.603495251; mid_quality=71.5670103093; avg_quality=66.9914529915; seq_a_single=8; score_norm=3.91686628961; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacagcaacaaatgtccataacacccaagcactgacaaggggagccaagtc +gtaa +>11_19926/1_CONS_SUB_SUB ali_length=108; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=423.575366834; mid_quality=71.5961538462; avg_quality=67.2741935484; seq_a_single=8; score_norm=3.92199413735; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaaatcgtaa +>11_20015/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=367.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.75123377627; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_20292/1_CONS_SUB_SUB reverse_score=80.0; merged_sample={'S15-11': 4}; direction=forward; seq_a_insertion=0; seq_b_deletion=1; forward_score=68.0; reverse_match=cttccggtacacttaccatg; seq_length_ori=123; seq_a_deletion=0; count=4; seq_length=70; reverse_tag=attcgcag; experiment=S15-11; status=full; mode=alignment; forward_tag=attcgcag; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; seq_a_single=8; head_quality=44.8; +cccctcacacatcaaatcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_20413/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggtgacaaggggaggcaagtcgt +aa +>11_20545/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=71; status=full; mode=alignment; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_20588/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacatcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_20599/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggccggggag +gcaagtcgtaa +>11_20611/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=371.624245684; mid_quality=70.8631578947; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatcacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_20627/1_CONS_SUB_SUB ali_length=108; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=399.595434532; mid_quality=70.6153846154; avg_quality=66.4516129032; seq_a_single=8; score_norm=3.69995772715; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaaattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_20700/1_CONS_SUB_SUB ali_length=102; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=118; reverse_tag=attcgcag; count=3; seq_length=65; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccaagcaactggacctaaaattttctttaaaccctaacaaccgcgaaggagaggaaagt +cgtaa +>11_20741/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaaagtcg +taa +>11_20783/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.597566785; mid_quality=71.4947368421; avg_quality=66.852173913; seq_a_single=8; score_norm=3.91512693723; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgagcaaaggggaggcaagtcgt +aa +>11_20896/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_21052/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcactataaaaataactaataatataacactaattaaggggagataagtcgt +aa +>11_21406/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacactactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_21577/1_CONS_SUB_SUB ali_length=98; seq_ab_match=98; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=391.606202164; mid_quality=72.0; avg_quality=67.2280701754; seq_a_single=8; score_norm=3.99598165473; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgagaggaggcaagtcgta +a +>11_21623/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=41.2; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgaactcaaaccaaacattcttaattaaacatttatcagcacagtcgt +aa +>11_21758/1_CONS_SUB_SUB ali_length=102; seq_ab_match=102; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=399.590128783; mid_quality=72.0; avg_quality=67.3898305085; seq_a_single=8; score_norm=3.91755028218; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=118; reverse_tag=attcgcag; count=1; seq_length=65; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacaacctatcttaagccccgttcaatacgcacaaggggaggaaagt +cgtaa +>11_21794/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagctaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_21909/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctctataatataacactaattaaggggagataagtcgt +aa +>11_22064/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.605519428; mid_quality=71.625; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgtcacacagcaacaaatgtccataacacccaagcactgacaaggggaggcaagtcg +taa +>11_22202/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttaaataaacacttcgatggcaaaggagagggaagtcgt +aa +>11_22212/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_22457/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcgactcgataaaatatattcttaaacaaagatagaactaaagaggaggcaagtcgta +a +>11_22825/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaagggggcaaagtcg +taa +>11_22932/1_CONS_SUB_SUB ali_length=107; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=395.592098922; mid_quality=70.9514563107; avg_quality=66.6991869919; seq_a_single=8; score_norm=3.69712241983; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacttcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_22996/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=71.3076923077; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaatatttatcagcacggggag +gcaagtcgtaa +>11_23215/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcgactcgataaaaatatattcttaaacaaagatagaactaaagaggaggcaagtcgt +acaagtcgtaa +>11_23372/1_CONS_SUB_SUB ali_length=108; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=2; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaatcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_23426/1_CONS_SUB_SUB ali_length=98; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=56.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcgactcgataaaaatatattcttaaacaaagatagaactaaagaggaggcaagtcgta +a +>11_23854/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaacaaggggaggcaagtcgt +aa +>11_23992/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_24127/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_24370/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaagggtaggcaagtcgt +aa +>11_24429/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.6170212766; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ctccgaattcacttaaccagaaatatctaatataatagcactgacaaggggaaagtcgta +a +>11_24528/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=371.624245684; mid_quality=70.8631578947; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattctttcgatggcaaaggagagggaagtcgt +aa +>11_24592/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=71.3076923077; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ccaagcttagtactaaacctaactaaaatcttaattaaacatttatcagcacggggaggc +aagtcgtaa +>11_24794/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620227339; mid_quality=71.25; avg_quality=66.3793103448; seq_a_single=8; score_norm=3.75620227339; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctcacacatcaaaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_24894/1_CONS_SUB_SUB ali_length=99; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=371.624245684; mid_quality=70.8631578947; avg_quality=66.3304347826; seq_a_single=8; score_norm=3.75378025943; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatgaggcaagtcgt +aa +>11_25015/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=41.2; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=72.0; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaataaggggagataagtcgta +a +>11_25157/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacaaaggagagggaagtcgt +aa +>11_25221/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaacactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_25263/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaataacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_25266/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaattaaggggagataagtcgt +gtaa +>11_25511/1_CONS_SUB_SUB ali_length=108; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=423.573372666; mid_quality=71.6538461538; avg_quality=67.3225806452; seq_a_single=8; score_norm=3.92197567283; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttttaaacatttatcagcacggggag +gcaagtcgtaa +>11_25596/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagcg +taa +>11_25796/1_CONS_SUB_SUB ali_length=102; seq_ab_match=100; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=56.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=383.604836693; avg_quality=66.7796610169; seq_a_single=8; score_norm=3.76083173228; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=118; reverse_tag=attcgcag; count=2; seq_length=65; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacactctatcttaagccccgttcaatacgcacaaggggaggaaagt +cgtaa +>11_25972/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=41.2; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatataaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_26006/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagaggcaagaggc +aagtcgtaa +>11_26023/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_26086/1_CONS_SUB_SUB ali_length=108; seq_ab_match=103; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=3; score=391.602788487; mid_quality=70.2692307692; avg_quality=66.1612903226; seq_a_single=8; score_norm=3.62595174525; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_26103/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgta +>11_26454/1_CONS_SUB_SUB ali_length=108; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=399.595434532; mid_quality=70.9615384615; avg_quality=66.4516129032; seq_a_single=8; score_norm=3.69995772715; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +ccccgacatcacagcactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_26838/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_26848/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=371.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.75370597706; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagataagtaaatcttttcaggaacaaaggggaggcaagtcgt +aa +>11_26880/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_27026/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacagcaacaaatgtccataacacccaagcactaagaggaggcaagtcgtt +aa +>11_27255/1_CONS_SUB_SUB ali_length=108; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=399.595434532; mid_quality=70.6153846154; avg_quality=66.4516129032; seq_a_single=8; score_norm=3.69995772715; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aacatgggtaa +>11_27366/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_27613/1_CONS_SUB_SUB ali_length=108; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=423.573372666; mid_quality=71.6538461538; avg_quality=67.3225806452; seq_a_single=8; score_norm=3.92197567283; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacttcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_27820/1_CONS_SUB_SUB ali_length=102; seq_ab_match=101; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=1; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=391.597482738; mid_quality=71.6326530612; avg_quality=67.0847457627; seq_a_single=8; score_norm=3.83919100723; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=118; reverse_tag=attcgcag; count=1; seq_length=65; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacaactctacttaagccccgttcaatacgcacaaggggaggaaagt +cgtaa +>11_27913/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaaaacttaacaccaagaggaggcaagtcgta +a +>11_28016/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtataaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_28312/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctatataatataacactaattaaggggagataagtcgt +aa +>11_28385/1_CONS_SUB_SUB ali_length=100; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=399.598165473; mid_quality=72.0; avg_quality=67.3103448276; seq_a_single=8; score_norm=3.99598165473; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgaggaacaaaggggaggcaagtcgta +a +>11_28442/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgtaa +>11_28782/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_28829/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.612873383; mid_quality=71.625; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.75612873383; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctgtaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_29371/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.615550287; mid_quality=71.5531914894; avg_quality=66.8596491228; seq_a_single=8; score_norm=3.91444439068; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaacaagaggaggcaagtcgta +a +>11_29436/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttaaataaacaataatccaggataaggggaggaagtcgt +aa +>11_29471/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagagagagggaagtgta +a +>11_29485/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_29568/1_CONS_SUB_SUB ali_length=107; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=403.584744967; mid_quality=71.3009708738; avg_quality=66.9918699187; seq_a_single=8; score_norm=3.77182004642; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttacagcacggggagg +caagtcgtaa +>11_29575/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_29843/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttaggaacaaaggggaggcaagtcgt +aa +>11_29911/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=68.0; direction=forward; seq_b_insertion=0; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_29972/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=62.0; forward_tag=attcgcag; seq_b_mismatch=1; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgacaaaggggaggcaagtcgta +a +>11_29980/1_CONS_SUB_SUB ali_length=101; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616208993; mid_quality=70.8865979381; avg_quality=66.4273504274; seq_a_single=8; score_norm=3.75857632667; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_30114/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattcaccaagaggaggcaagtcgta +a +>11_30167/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacagcatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_30239/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=367.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.75123377627; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatcataaaaaaacttaacaccaagaggaggcaagggggta +a +>11_30287/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacaactctatcttaagccccgttcaatacgcacaaggggaggggag +gcaagtcgtaa +>11_30518/1_CONS_SUB_SUB ali_length=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaataaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcgt +aa +>11_30755/1_CONS_SUB_SUB ali_length=108; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=423.561401678; mid_quality=71.5384615385; avg_quality=67.2258064516; seq_a_single=8; score_norm=3.92186483035; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactccaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_30994/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.611531941; mid_quality=71.5578947368; avg_quality=66.9043478261; seq_a_single=8; score_norm=3.91526799941; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgcaa +>11_31115/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaacaaaggggaggcaagtcgt +aa +>11_31265/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactcgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcgt +aa +>11_31518/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=41.2; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.605519428; mid_quality=72.0; avg_quality=67.0; seq_a_single=8; score_norm=3.83605519428; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_31560/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaacgcataagaaatacataaccaaccaatggcaaaggagagggaagtcgt +aa +>11_31690/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.611531941; mid_quality=71.5578947368; avg_quality=66.9043478261; seq_a_single=8; score_norm=3.91526799941; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttctatggcaaaggagagggaagtcgtaa +>11_32407/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacaacaaggggaggcaagtc +gtaa +>11_32446/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatt; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gt +>11_32504/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=2; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaagcttcgatggcaaaggagagggaagtcgt +aa +>11_32552/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 3}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=3; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_32574/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggggaagtcgt +aa +>11_32681/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=391.59354844; mid_quality=71.5; avg_quality=66.8965517241; seq_a_single=8; score_norm=3.9159354844; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacataggggaggcaagtcg +taa +>11_32797/1_CONS_SUB_SUB ali_length=101; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=379.616208993; mid_quality=70.8865979381; avg_quality=66.4273504274; seq_a_single=8; score_norm=3.75857632667; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatcagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_32868/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_32897/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtacttctggttcttaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_33317/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=375.620227339; mid_quality=70.875; avg_quality=66.3793103448; seq_a_single=8; score_norm=3.75620227339; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataaataaacaataatccaggataaggggaggaaagtcg +taa +>11_33619/1_CONS_SUB_SUB ali_length=103; seq_ab_match=101; tail_quality=41.2; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=395.600818348; mid_quality=71.6363636364; avg_quality=66.8235294118; seq_a_single=8; score_norm=3.84078464415; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=119; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtat +aaca +>11_33816/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccactt; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=72.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaacacggt +>11_33950/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtacaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_34117/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=1; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cttcaaaaccacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_34336/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.611531941; mid_quality=71.5578947368; avg_quality=66.9043478261; seq_a_single=8; score_norm=3.91526799941; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaattaacgaataaaaataactaataatataacactaattaaggggagataagtcgt +aa +>11_34586/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaataaacgaataaaaataactaataatataacactaattaaggggagataagtcgtaa +>11_34930/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttaaataaacaataatccaggataaggggaggaaagtcg +taa +>11_35205/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacataatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_35285/1_CONS_SUB_SUB ali_length=107; seq_ab_match=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=419.570037056; mid_quality=72.0; avg_quality=67.5772357724; seq_a_single=8; score_norm=3.92121529959; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacatcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_35320/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.6210526316; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=41.2; seq_b_single=8; +cccctcacttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_35423/1_CONS_SUB_SUB ali_length=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=2; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccttcaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtcg +taa +>11_35426/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggggcaagtcgt +aa +>11_35549/1_CONS_SUB_SUB ali_length=97; seq_ab_match=96; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=371.617574464; mid_quality=71.6129032258; avg_quality=66.8672566372; seq_a_single=8; score_norm=3.83110901509; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=113; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccaagtttaacaccttttaaataaaaaattaacagaacaaaggagaggcaagtcgtaa +>11_35765/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcggt +aa +>11_35887/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcagttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_36016/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.618885897; mid_quality=71.1789473684; avg_quality=66.5913043478; seq_a_single=8; score_norm=3.83453420098; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaatttacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_36309/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttaaataaacaatatccaggataaggggaggaaagtcgt +aa +>11_36453/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.615550287; mid_quality=71.5531914894; avg_quality=66.8596491228; seq_a_single=8; score_norm=3.91444439068; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaagacttaacaccaagaggaggcaagtcgta +a +>11_36533/1_CONS_SUB_SUB ali_length=107; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; forward_tag=attcgcag; seq_b_mismatch=2; experiment=S15-11; seq_a_single=8; reverse_score=80.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=2; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaacaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_36551/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=71.3076923077; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_36787/1_CONS_SUB_SUB ali_length=101; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=379.616208993; mid_quality=70.8865979381; avg_quality=66.4273504274; seq_a_single=8; score_norm=3.75857632667; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaataaaacgcataagaaatacataaccaaccagcactgacaaggggaggcaagtc +gtaa +>11_36794/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaacaacattcttaattaaacatttatcagcacggggaggc +aagtcgtaa +>11_36821/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=1; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=371.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.75370597706; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcgaagtcgt +aa +>11_37045/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaaataaactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_37074/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.568755633; mid_quality=71.1923076923; avg_quality=66.935483871; seq_a_single=8; score_norm=3.84785884846; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacaatcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_37207/1_CONS_SUB_SUB ali_length=103; seq_ab_match=101; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=395.600818348; mid_quality=71.2727272727; avg_quality=66.8235294118; seq_a_single=8; score_norm=3.84078464415; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=119; reverse_tag=attcgcag; count=1; seq_length=66; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaacacaaggggaggaaag +tcgtaa +>11_37251/1_CONS_SUB_SUB ali_length=100; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=391.605519428; mid_quality=71.625; avg_quality=67.0; seq_a_single=8; score_norm=3.91605519428; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctgcataagtaaatcttttcaggaacaaaggggaggcaagtcg +taa +>11_37347/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_37424/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttacgatggcaaaggagagggaagtcgt +aa +>11_37451/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=2; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgcatagcggataaattttaaataaacaataatccaggataaggggaggaaagtcgt +aa +>11_37500/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccaagcttagtactaaacctaactaaaaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_37520/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=41.2; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.590074744; mid_quality=71.25; avg_quality=66.6935483871; seq_a_single=8; score_norm=3.77398217356; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacgaggag +gcaagtcgtaa +>11_37717/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=371.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.75370597706; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccacatagcggataaattttaaataaacaataatccaggataaggggaggaaagtcgt +aa +>11_37973/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +acaagtcgtaa +>11_38039/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=395.603495251; mid_quality=71.5670103093; avg_quality=66.9914529915; seq_a_single=8; score_norm=3.91686628961; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcccccagacagccgtaattaacaaccctgacatgtgcgaaggggaggaaagtc +gtaa +>11_38069/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaattaaacattggggaggcaagtcgt +aa +>11_38172/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaaaaaatgcaccagaaatatctaatataatagcactgacaaggggaggcaagtc +gtaa +>11_38246/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacaactctatcttaagccccgttcaatacgcagggaggcaagtcgt +aa +>11_38331/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatgcaaaggagagggaagtcgtaa +>11_38433/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaacgaacaaaggggaggcaagtcgta +a +>11_38712/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_38775/1_CONS_SUB_SUB ali_length=101; seq_ab_match=101; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=403.594147128; mid_quality=72.0; avg_quality=67.3504273504; seq_a_single=8; score_norm=3.99598165473; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaattaaggggagataagtcgt +aaca +>11_39263/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcgactcgataaaaatatattcttaacaaagatagaactaaagaggaggcaagtcgta +a +>11_39561/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaagttcttataatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_39822/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=375.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.83279138897; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatcggttcttaacaagacaaccgaacaaaggggaggcaagtcgta +a +>11_39936/1_CONS_SUB_SUB ali_length=107; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=411.577391011; mid_quality=71.6504854369; avg_quality=67.2845528455; seq_a_single=8; score_norm=3.846517673; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacacaaactcaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_40113/1_CONS_SUB_SUB ali_length=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 2}; forward_score=64.0; forward_tag=attcgcag; seq_b_mismatch=2; experiment=S15-11; seq_a_single=8; reverse_score=74.0; direction=forward; seq_b_insertion=0; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=2; seq_length=59; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctactaaaacgcttcgatggcaaaggagagggaagtcgtaa +>11_40293/1_CONS_SUB_SUB ali_length=101; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=379.616208993; mid_quality=70.8865979381; avg_quality=66.4273504274; seq_a_single=8; score_norm=3.75857632667; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactataatataatagcactgacaaggggaggcaagtcgt +aa +>11_40357/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatc; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cctcaaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +acatggtaagt +>11_40390/1_CONS_SUB_SUB ali_length=99; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=395.602183818; mid_quality=72.0; avg_quality=67.2695652174; seq_a_single=8; score_norm=3.99598165473; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_40421/1_CONS_SUB_SUB ali_length=108; seq_ab_match=106; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=415.580726621; mid_quality=71.3076923077; avg_quality=67.0322580645; seq_a_single=8; score_norm=3.84796969094; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=69; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaaacacatcaaactcaaaccaaacattcttaattaaacatttatcagcacggggaggc +aagtcgtaa +>11_40429/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=41.2; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.6288659794; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggggggaggcaagtcgt +aa +>11_40451/1_CONS_SUB_SUB ali_length=108; seq_ab_match=105; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=407.588080577; mid_quality=70.9615384615; avg_quality=66.7419354839; seq_a_single=8; score_norm=3.77396370904; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcacacagcaacaaatgtccataacacccaagcactgtttatcagcacggggag +gcaagtcgtaa +>11_40519/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagactcgatggcaaaggagagggaagtcgt +aa +>11_40529/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=agcttcctacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=None; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtacaatgaagttacctaacaccaaagctctgacaaggggaggcaagtcgt +aa +>11_41020/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaacaagacaaccgaacaaaggggaggcaagtcgtaa +>11_41180/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=2; score=375.620227339; mid_quality=70.875; avg_quality=66.3793103448; seq_a_single=8; score_norm=3.75620227339; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaaaggagagggaaagtcgta +a +>11_41182/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=395.589530095; mid_quality=71.5051546392; avg_quality=66.9401709402; seq_a_single=8; score_norm=3.91672802074; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaatgcaccagaaatatctaatataatagcactgacaacgggaggcaagtc +gtaa +>11_41522/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtct +aa +>11_41679/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaattaaggggagataagtcgt +aa +>11_41839/1_CONS_SUB_SUB ali_length=101; seq_ab_match=100; tail_quality=44.8; reverse_match=cttccggtacacttaccgtg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=395.601501083; mid_quality=71.6288659794; avg_quality=67.0427350427; seq_a_single=8; score_norm=3.91684654538; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaagggggaggcaagtc +gtaa +>11_41845/1_CONS_SUB_SUB ali_length=107; seq_ab_match=103; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=387.599452877; mid_quality=70.6019417476; avg_quality=66.406504065; seq_a_single=8; score_norm=3.62242479324; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaactcaaaccaaacattcttattaaacatttatcagcacggggagg +caagtcgtaa +>11_42085/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggaagtcgt +aa +>11_42120/1_CONS_SUB_SUB ali_length=108; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=399.595434532; mid_quality=70.6153846154; avg_quality=66.4516129032; seq_a_single=8; score_norm=3.69995772715; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=124; reverse_tag=attcgcag; count=1; seq_length=71; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaaataaactcaaaccaaacattcttaattaaacatttatcagcacggggag +gcaagtcgtaa +>11_42163/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcgctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccaataaacgaataaaaataactaataatataacactaattaaggggagggaagtcgt +aa +>11_42398/1_CONS_SUB_SUB ali_length=107; seq_ab_match=104; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=395.592098922; mid_quality=70.9514563107; avg_quality=66.6991869919; seq_a_single=8; score_norm=3.69712241983; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=123; reverse_tag=attcgcag; count=1; seq_length=70; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacatcaaaccaaaccaaacattcttaattaaacatttatcagcacggggagg +caagtcgtaa +>11_42611/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctcctcacacatcaaactacctaactaaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_42997/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttaacaccaagaggaggcaagtcgta +a +>11_43023/1_CONS_SUB_SUB ali_length=98; seq_ab_match=96; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620910074; mid_quality=71.2340425532; avg_quality=66.5964912281; seq_a_single=8; score_norm=3.83286642933; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaaggggaggcaagtcg +a +>11_43141/1_CONS_SUB_SUB ali_length=101; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=387.608855038; mid_quality=71.2577319588; avg_quality=66.735042735; seq_a_single=8; score_norm=3.83771143602; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=117; reverse_tag=attcgcag; count=1; seq_length=64; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctcacacacagcaacaaatgtccataacacccaagcactgacaaggggaggcaagtc +gtaa +>11_43169/1_CONS_SUB_SUB ali_length=100; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=375.620227339; mid_quality=70.875; avg_quality=66.3793103448; seq_a_single=8; score_norm=3.75620227339; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacaaccaaacaacaataatccaggataaggggaggaaagtcgta +a +>11_43329/1_CONS_SUB_SUB ali_length=98; seq_ab_match=97; tail_quality=44.8; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=383.613556119; mid_quality=71.6170212766; avg_quality=66.9122807018; seq_a_single=8; score_norm=3.91442404203; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgaaacaacccaataagatctataaaaaaacttaacaccaagaggaggcaagtcgta +a +>11_43478/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccaatacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaaaatataacactaattaaggggagataagtcgt +aa +>11_43493/1_CONS_SUB_SUB ali_length=99; seq_ab_match=99; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=0; score=395.59021283; mid_quality=71.8736842105; avg_quality=67.1652173913; seq_a_single=8; score_norm=3.99586073566; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagataaccgaacaaaggggaggcaagtcgt +aa +>11_43769/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcaccct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=56.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +caccaagcatctgaacaactctatcttacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_43992/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcaccctct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=64.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=60; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccaagcttagtactaaacctaactaaaacgcttcgatggcaattatcaggcaagtcgtaa +>11_44039/1_CONS_SUB_SUB ali_length=100; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=383.612873383; mid_quality=71.25; avg_quality=66.6896551724; seq_a_single=8; score_norm=3.83612873383; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=116; reverse_tag=attcgcag; count=1; seq_length=63; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +cccctgtcaaatgcaataaagataaataataccaaagcgctgacaaggggaggcaagtcg +taa +>11_44084/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=0; score=387.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.9152478563; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccccattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagtcgt +aa +>11_44097/1_CONS_SUB_SUB ali_length=98; seq_ab_match=94; tail_quality=37.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=2; score=359.633621823; mid_quality=70.8631578947; avg_quality=65.7217391304; seq_a_single=8; score_norm=3.66973083493; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=9; +cccctcacacatcaaactcaaaccataaaacgcttcgatggcaaaggagagggaagtcgt +aa +>11_44172/1_CONS_SUB_SUB ali_length=99; seq_ab_match=98; tail_quality=44.8; reverse_match=cttccggtacacttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=0; forward_tag=attcgcag; seq_b_mismatch=1; score=379.609537774; mid_quality=71.6210526316; avg_quality=66.9565217391; seq_a_single=8; score_norm=3.83443977549; reverse_score=80.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=1; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgactccgaactaaaagcataagtaaatcttttcaggaacaaggggaggcaagtcgt +aa +>11_44223/1_CONS_SUB_SUB ali_length=99; seq_ab_match=97; tail_quality=44.8; reverse_match=cttccggtacgcttaccatg; seq_a_deletion=0; forward_match=acaccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=68.0; seq_a_mismatch=1; forward_tag=attcgcag; seq_b_mismatch=1; score=379.616891729; mid_quality=71.2421052632; avg_quality=66.6434782609; seq_a_single=8; score_norm=3.83451405787; reverse_score=74.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=115; reverse_tag=attcgcag; count=1; seq_length=62; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ccccgacatagcggataaattttaaataaacaataatccaggataaggggaggaaagtcg +ta +>11_44824/1_CONS_SUB_SUB ali_length=98; seq_ab_match=95; tail_quality=41.2; reverse_match=tttccagtacacttaccatg; seq_a_deletion=0; forward_match=ataccgcccgtcactct; forward_primer=acaccgcccgtcactct; reverse_primer=cttccggtacacttaccatg; merged_sample={'S15-11': 1}; forward_score=62.0; seq_a_mismatch=2; forward_tag=attcgcag; seq_b_mismatch=1; score=367.628264029; mid_quality=71.2340425532; avg_quality=66.2807017544; seq_a_single=8; score_norm=3.75130881662; reverse_score=68.0; direction=forward; seq_b_insertion=0; experiment=S15-11; seq_b_deletion=0; seq_a_insertion=0; seq_length_ori=114; reverse_tag=attcgcag; count=1; seq_length=61; status=full; mode=alignment; head_quality=44.8; seq_b_single=8; +ctccgaattcacttaatctggttcttaacaagacaaccgaacaaaggggaggcaagcgta +a diff --git a/04_filtering/test/test_filter_prinseq.sh b/04_filtering/test/test_filter_prinseq.sh new file mode 100644 index 0000000000000000000000000000000000000000..4d3e669ca87dd11cb117ff5381cad789e34190b0 --- /dev/null +++ b/04_filtering/test/test_filter_prinseq.sh @@ -0,0 +1,12 @@ +source 98_infos/config.sh +prinseq=${SINGULARITY_EXEC_CMD}" "${EDNATOOLS_SIMG}" perl prinseq-lite-0.20.4/prinseq-lite.pl" +obiclean=${SINGULARITY_EXEC_CMD}" "${OBITOOLS_SIMG}" obiclean" + + +dereplicated_sample=`pwd`"/04_filtering/test/04_prinseq/grinder_teleo1_sample_S15-11.uniq.l20.fasta" +good_sequence_sample=`pwd`"/04_filtering/test/04_prinseq/OUT_PRINTSEQ" + +$prinseq -fasta "${dereplicated_sample}" -min_len 20 -ns_max_n 0 -noniupac --out_format 1 -out_good "${good_sequence_sample}" + +clean_sequence_sample="${good_sequence_sample}".r005.clean.fasta +$obiclean -r 0.05 -H "${good_sequence_sample}".fasta > "${clean_sequence_sample}" \ No newline at end of file